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About the workshop
This workshop will cover entire array tomography (AT) workflows including sample preparation, serial ultramicrotomy, light and electron microscopy, and image analysis. Practicals include traditional AT methods like rigid support AT and automated tape collecting ultramicrotomy (ATUM), correlative and conjugate light and electron microscopy. Novel technologies will also be discussed. |
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Location
DZNE Munich, Germany
Organizers
Imaging Scientists from the Chan Zuckerberg Initiative (CZI) "Array Tomography Network and Training"
Format
5 days hands-on course and lectures on array tomography (AT) techniques.
Who
20 participants with basic to expert knowledge in imaging (either light or electron microscopy).
Content
sample preparation, serial sectioning, solid support AT, ATUM, CLEM, IF-AT, serial section mapping and imaging (light and electron microscopy), image analysis
Instructors
CZI AT team
Jemima Burden, Bruno Humbel, Naomi Kamasawa, Kristina Micheva, Thomas Misgeld, Josh Morgan, Martina Schifferer, Mariano Soiza-Reilly and Mark Terasaki
Experts from research and commercial sectors
Helmut Gnägi (Diatome), Richard Schalek, Anna-Lena Eberle (Zeiss), Tilman Franke (Thermo Fisher Scientific), Gareth Morgan (RMC), tbd
Schedule
Welcome evening: 3 November 2024;
Workshop: 4 - 8 November 2024
Morning: talks, industry lectures, demos.
Afternoon: rotating hands-on practicals. Details to follow shortly
Fee, accommodation
400 € (this includes 6 nights, single room accommodation with breakfast, at a CZI subsidized rate)
Travel
to be paid by participant, CZI-sponsored travel grants are available (please indicate in your application)
Application Deadline
31 May 2024 - CLOSED.
Selection process
CZI AT team selects participants according to the motivation letter and, if appropriate, the reference, respecting diversity criteria. Chosen participants will be notified by 28 June 2024
Selection criteria
experience in ultramicrotomy and/or imaging, expected impact of AT training on the applicant’s current and future career goals, expected impact of AT training on local and global scientific environment and knowledge dissemination, diversity of biological fields of application
Child care support
available (please indicate in your application): Eligible costs include fees for caregiver or for taking the child to the meeting etc.. Please indicate in your application if you would like to be considered for the grant and how you intend to use the grant including the sum that you will need.
Administration
Congress Center LMU (Sabine Beutlhauser, Philine Puntsch [email protected])
Program
Sunday, November 3rd
17:00-18:00 |
Registration |
all |
Rooftop-Lounge (G2B) Campus At Home |
18:00-18:30 |
Welcome |
organizers |
Rooftop-Lounge (G2B) Campus At Home |
18:30 |
Attendee introduction and reception |
all |
Rooftop-Lounge (G2B) Campus At Home |
Monday, November 4th
8:30-9:30 |
Serial ultramicrotomy |
Helmut Gnägi |
Innovations- und Gründerzentrum Biotechnologie (IZB) seminar room |
9:30-10:00 |
Tape vs. solid support AT |
Jemima Burden, Josh Morgan |
IZB seminar room |
10:00-10:30 |
Coffee break |
all |
IZB seminar room |
10:30-11:30 |
ATUM & large scale AT & automated microscopy |
Richard Schalek |
IZB seminar room |
11:30 |
Lunch boxes |
all |
IZB seminar room |
12:00-17:30 |
Rotating practicals with flexible lunch and coffee break |
all |
German Center for Neurodegenerative Diseases (DZNE) |
17:45 |
Departure for evening event |
all |
DZNE entrance |
Tuesday, November 5th
9:00-9:30 |
Sample preparation |
Bruno Humbel |
IZB seminar room |
9:30-10:00 |
AT for studying tissue organisation |
Mark Terasaki |
IZB seminar room |
10:00-10:30 |
Coffee break |
all |
IZB seminar room |
10:30-11:00 |
Multimodal AT |
Martina Schifferer |
IZB seminar room |
11:00-12:00 |
Zeiss industry talk MultiSEM, sense BSD |
Anna Lena Eberle |
IZB seminar room |
12:15-13:00 |
Lunch |
all |
Seven and More |
13:15-18:15 |
Rotating practicals with flexible coffee break |
all |
DZNE |
Wednesday, November 6th
9:00-9:30 |
CLEM using AT |
Jemima Burden |
IZB seminar room |
9:30-10:00 |
ATUM vs SBF-SEM for CLEM in neuroscience |
Naomi Kamasawa |
IZB seminar room |
10:00-10:30 |
Coffee break |
all |
IZB seminar room |
10:30-11:00 |
CLEM to study axon loss |
Thomas Misgeld |
IZB seminar room |
11:00-11:30 |
IF-AT vs IF+SEM to study synapses |
Kristina Micheva |
IZB seminar room |
11:30-12:00 |
IF-AT to study input-specific brain circuits |
Mariano Soiza-Reilly |
IZB seminar room |
12:00-12:45 |
Easy CLEM |
Dumisile Lumkwana |
IZB seminar room |
13:00-13:45 |
Lunch |
all |
Seven and More |
14:00-19:00 |
Rotating practicals with flexible coffee break |
all |
DZNE |
Thursday, November 7th
9:00-10:00 |
Image analysis for AT overview |
Jemima Burden, Josh Morgan |
IZB seminar room |
10:00-10:30 |
Coffee Break |
all |
IZB seminar room |
10:30-11:00 |
Commercial image analysis solution |
Norman Rzepka |
IZB seminar room |
11:00 |
Lunch boxes |
all |
IZB seminar room |
11:30-17:00 |
Rotating practicals with flexible lunch and coffee break |
all |
DZNE |
17:15 |
Departure for evening event |
all |
DZNE entrance |
Friday, November 8th
9:00-9:45 |
Deep learning-based image analysis |
Yusuke Hirabayashi (remote) |
IZB seminar room |
9:45-10:30 |
ATUM tape & grid tape |
Yoshiyuki Kubota (remote) |
IZB seminar room |
10:30-11:00 |
Coffee Break |
all |
IZB seminar room |
11:00-11:45 |
GAUSS-EM |
Kevin Briggman |
IZB seminar room |
11:45-12:30 |
MagC &CLEM, remote |
Thomas Templier (remote) |
IZB seminar room |
12:30-13:15 |
Lunch |
all |
IZB seminar room |
13:30-15:30 |
Attendee next steps, lessons learned, feedback |
all |
IZB seminar room |
15:30-16:00 |
Coffee Break |
all |
IZB seminar room |
16:00-17:00 |
Attendee panel discussion |
all |
IZB seminar room |
17:00-17:30 |
Wrap-Up |
Organizers |
IZB seminar room |
Sponsors
SyNergy (Munich Cluster for Systems Neurology), Thermo Fisher Scientific, Zeiss, Leica, Diatome, RMC Boeckeler, Science Services
SyNergy (Munich Cluster for Systems Neurology), Thermo Fisher Scientific, Zeiss, Leica, Diatome, RMC Boeckeler, Science Services
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