ATUM.

= Automated Tape Collecting Ultramicrotomy

Principle ATUM

Serial section collection onto plastic tape.

Publications ATUM

First method description and applications

• Schalek_Microsc Micoranal_2011
• Terasaki_Cell_2013
• Kasthuri_Cell_2015​
• Webster_MicroscAnal_2015 atumtome_microscopyanalysismarchapril_2015.pdf 

Method paper on the ATUM approach

• ​Baena_MCB_2019
• Kislinger_MCB_2023

ImmunoEM and ATUM

• Post-embedding immunogold: Norris_Terasaki 2017
• Post-embedding immunogold: Norris&Terasaki 2021
• Pre-embedding immunogold: Baena_Jaffe 2020

Carbon nanotube tape

• Kubota_Nat Commun_2018

ATUM applications - connectomics (large scale)

• ​Morgan_Cell-2016
• ​Hildebrand_Nature_2017
• ​Shapson-Coe_bioRxiv_2021
• Witvliet_Nature_2021

ATUM applications - small/medium scale

• ​Thomas_J Neurosci_2019
• ​Terasaki_Sci Rep_2020
• ​Joesch_eLIFE_2016

ATUM CLEM

• ​Thomas_Microsc Microanal_2021
• ​FriedrichsesN_Front_2022
• ​Snaidero_Nat Commun_2020
• Sun_JNeurosci_2021
• Sun_Neuromet_2020

Wafer Mapping

• Hayworth_Front Neural Circuits_2014

Technical variants

​Larsen_Commun Biol_2021
​Li_J Struct Biol_2017

Resources ATUM

Building a tape plasma discharger

pelcoeasiglow.pdf
File Size:	2333 kb
File Type:	pdf

Download File

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Tutorial movies ATUM

Jeff Lichtman - Connectomics, ATUM principle

RMC Boeckeler - ATUMtome principle, settings

Mathieu Legrand, Eden Instruments - ATUMtome sectioning, wafer mounting

Vendor information ATUM

RMC Bockeler ATUMtome

Image gallery ATUM