Principle ssAT.

Serial sections are collected onto solid support, e.g. coated glass or silicon wafers or microscope slides..

Publications ssAT.

First method description and discussion

• Micheva_Smith_2007
• Micheva_2010
• Smith_BMCBIOL_2018

Method paper and protocols

• White-Burden_MCB_2023
• Micheva_CSHprotocols_2010
• Wacker_J Microsc_2013
• Kay_Nat Prot_2013
• Markert_MCB_2017
• Micheva_Cellul Imaging_2017
• Franke_Jove_2021​
• Kolotuev_JOM_2023

Light microscopy ssAT

• Immunofluorescence AT

Electron microscopy ssAT

- resources coming soon...

Conjugate ssAT

CLEM

• Micheva_Elife_2016
• Collmann_J Neurosci_2015
• ​Markert_Neurophoton_2016

• Han_bioRxiv_2023
• Fang_NatMet_2018

New devices for ribbon collection

• Koike_ACAHistoCyto_2017
  
• Burel_Dev_2018

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Resources ssAT.

Tutorial movies ssAT.

Leica Artos principle

RMC Bockeler - ASH2 principle

Watch Video

Burden / White and Furzeland (Zeiss): From 3D light to 3D EM conference​

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Watch Video

Zeiss: ​Improving BSE detection for AT

White (Zeiss): Practical guide to starting SEM AT - online talk hosted by "BioEM Talks"​

Watch Video

Franke/Kolotuev: Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy​

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Moore: ​Low-cost solution for AT microtome manipulators - online talk hosted by "BioEM Talks"

Gnägi: Sectioning - online talk hosted by "BioEM Talks"

Commercial providers ssAT equipment

Leica ARTOS 3D

Leica ARTOS 3D

RMC ASH2

RMC AHS2

ATS diamond knife

ATS diamond knife